morphological study of the effects of X-rays on the melanocytes of the skin, under light microscope

To observe the effects of x-irradiation on the melanocytes of the skin under light microscope. A prospective experimental study. The study was carried out at Department of Anatomy, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre Karachi from 2008 to 2009. Thirty animals were taken and were divided in to two groups. Each group was further subdivided into three subgroups containing five animals each according to the time of sacrifice i.e. 48 hours, 15[th] day and 45[th] day respectively. A single whole body x-irradiation in a dose of 5 Gy was given. Animals were sacrificed under ether anesthesia after completion of their respective periods. Tissues were processed and 4-5 micron thick paraffin embedded sections were cut and stained with Masson's Fontana stain. Insignificant [P>0.05] decrease in number of melanocytes was obtained when compared to control in group B1 animals. In group B2, increase in size and number of melanocytes was noted with long dendritic processes and large amount of melanin seen scattered between the layers of epidermis was present and results were moderately significant [P<0.01] in case of face and abdomen and significant [P<0.05] in case of back. In group B3 Size and number of melanocytes were also increased with large amount of melanin scattered between the layers of epidermis. The results obtained were highly significant [P<0.001] in case of face and moderately significant [P<0.01] in case of abdomen and back. X-irradiated skin of face, abdomen and back of the guinea pig depicted increased number and size of melanocytes with long dendritic processes and large amount of melanin scattered between the layers of epidermis

effects of x-rays on the hair follicles, blood vessels, collagen and elastic fibers, of the skin of guinea pigs and the role of vitamin C, a morphological study under light microscope

To observe the effects of x-rays on hair follicles, blood vessels and collagen and elastic fibers of the skin and role of vitamin C. A prospective experimental study. This study was conducted at the Department of Anatomy, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre Karachi from 2008 to 2009. Twenty seven animals were taken and were divided in to three groups. Each group was further subdivided into three subgroups containing three animals each according to the time of sacrifice i.e. 48 hours, 15[th] day and 45 [th] day respectively. A single whole body x-irradiation in a dose of 5 Gy was given. Group C animals were also given injection of vitamin-C intraperitoneally in the dose of 1mg/G/day. Animals were sacrificed under either anaesthesia after completion of their respective periods. Tissues were processed and 4-5 micron thick paraffin embedded sections were cut and stained with Hematoxylin and eosin for morphology of hair follicles and blood vessels, Masson's trichrome for study of collagen and Van Geison for elastic fibers. Group A served as control. In Group 'B' hair follicles were reduced in size and number of cells per bulb was also reduced, blood vessels were dilated their endothelial cells were swollen lumen was narrow and vessel wall great extent in less than 15 days but there was no sign of improvement in elastic fibers. X-rays have hazardous effects on hair follicles, blood vessels and collagen fiber elastic fiber. Vitamin C minimizes these hazardous effects